First report of a subhypogeous basidiomycete, Scleroderma yunnanense (Boletales) from Japan
半地下生担子菌Scleroderma yunnanense (シラガニセショウロ, イグチ目) の日本初記録

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Taiga Kasuya, Kazumasa Imai, Kentaro Hosaka (2023) Truffology 6(1): 1–6
Submitted: 29 December 2022
Accepted: 17 March 2023
Published: 31 July 2023

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A subhypogeous sclerodermataceous fungus was collected in Nagoya, Aichi Prefecture, central Japan. Based on morphological observations and phylogenetic analyses using nuclear ribosomal DNA sequences, the present fungus was identified as Scleroderma yunnanense, belonging to Sclerodermataceae (Boletales). Scleroderma yunnanense is new to Japan and characterized by not stellately dehiscent basidiomata when mature, thick (2–7 mm) peridium covered by cream to pale yellowish scales, basidiomata covered with whitish mycelial strands, clamped hyphae, and globose to subglobose basidiospores densely covered with pyramidal warts.


愛知県名古屋市において、ニセショウロ型の半地下生菌の一種が採集された。本菌について、形態的特徴の観察および子実体より得られた核リボソームDNA の塩基配列 を用いた系統解析を行った。その結果、本菌はイグチ目ニセショウロ科に属するScleroderma yunnanense と同定された。本種は日本新産であり、子実体が成熟しても星形に裂開しない点、殻皮が厚く(2–7 mm)、クリーム色~淡黄色を帯びる鱗片に覆われる点、子実体の表面に白色の菌糸束が存在する点、菌糸にクランプ結合を有する点、そして担子胞子が球形~類球形で角錐状のいぼ状突起により密に覆われる点で特徴づけられる。本菌の和名をシラガニセショウロとする。


  1. Beaton G., Weste G. (1982) A new species of Scleroderma from Victoria, Australia. Transactions of the British Mycological Society 79: 41–43.
  2. Coccia M., Migliozzi V., Lavorato C. (1990) Studio sul genere Scleroderma Persoon. Bollettino dell’ Associazione Micologia ed Ecologica Romana 20–21: 3–59.
  3. Edgar R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797.
  4. Guzmán G. (1970) Monografia del género Scleroderma Pers. emend. Fr. (Fungi-Basidiomycetes). Darwiniana 16: 233–407.
  5. Hall T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  6. Kasuya T., Guzmán G. (2007) Veligaster nitidum, a pantropical sclerodermataceous fungus new to Japan and Thailand. Mycoscience 48: 259–262.
  7. Kasuya T., Guzmán G., Ramírez-Guillén F., Kato T. (2002) Scleroderma laeve (Gasteromycetes, Sclerodermatales), new to Japan. Mycoscience 43: 475–476.
  8. Kasuya T., Hosaka K., Uno K., Kakishima M. (2012) Phylogenetic placement of Geastrum melanocephalum and polyphyly of Geastrum triplex. Mycoscience 53: 411–426.
  9. Kasuya T., Uzawa M., Hosaka K. (2022) Scleroderma capeverdeanum, a subhypogeous fungus new to Japan, collected from the Eucalyptus plantation in Ibaraki Prefecture. Truffology 5: 23–32.
  10. Kumar S., Stecher G., Li M., Knyaz C., Tamura K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35: 1547–1549.
  11. Nouhra E.R., Caffot M.L.H., Pastor N., Crespo E.M. (2012) The species of Scleroderma from Argentina, including a new species from the Nothofagus forest. Mycologia 104: 488–495.
  12. Ortiz-Rivero J., Watling R., Guzmán-Dávalos L., Martín M.P. (2021) The many-rooted earthball—Scleroderma geaster and S. polyrhizum revisited, with the description of a new species. Phytotaxa 510: 1–17.
  13. Swofford D.L. (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods). MA Sinauer Associates, Sunderland.
  14. Tamura K. (1992) Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. Molecular Biology and Evolution 9: 678–687.
  15. Vilgalys R., Hester M. (1990) Rapid genetic identification and mapping of enzymatically amplified DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246.
  16. White T.J., Bruns T., Lee S., Taylor J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. (eds.) PCR protocols. Academic Press, New York, pp. 315–322.
  17. Zhang C., Xu X.-E., Liu J., He M., Wang W., Wang Y., Ji K. (2013) Scleroderma yunnanense, a new species from South China. Mycotaxon 125: 193–200.