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Orihara T, Castellano MA, Ohmae M, Kaneko Y, Hosaka K (2022) Truffology 5(1): 3–13
Submitted: 8 February 2022
Accepted: 21 March 2022
Published: 31 March 2022
Submitted: 8 February 2022
Accepted: 21 March 2022
Published: 31 March 2022
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Abstract
Two Japanese black deer truffles, Elaphomyces miyabeanus and E. nopporensis, were described from Hokkaido
in 1929 by Dr. Sanshi Imai. Since then, there have been no reliable, formal records of either species. Both species
were collected from the same locality on the same day, and are morphologically similar to each other. We
successfully located authentic specimens including syntypes of each species, which had been scattered and lost,
and we critically examined their morphology. We also collected fresh ascomata of E. miyabeanus / E. nopporensis
from the type locality and central Honshu. Based on examination of the rediscovered authentic specimens and
the fresh ascomata along with the original descriptions by Dr. Sanshi Imai, we concluded that E. nopporensis was
small, dried, old ascomata of E. miyabeanus and should be synonymized into the latter species. Phylogenetic
analyses based on the internal transcribed spacer region of the nuclear ribosomal DNA placed E. miyabeanus
within sect. Ceratogaster subsect. Sclerodermei along with E. anthracinus. The resultant tree also showed that E. miyabeanus was conspecific with Elaphomyces sp. collected in Norway, suggesting that the species is broadly
distributed across northern Eurasia.
References
- Anisimova M., Gascuel O. (2006) Approximate likelihood-ratio test
for branches: A fast, accurate, and powerful alternative. Systematic
Biology 55: 539–552. - Castellano M.A., Beever R.E., Trappe J.M. (2012a) Sequestrate
fungi of New Zealand: Elaphomyces (Ascomycota, Eurotiales,
Elaphomycetaceae). New Zealand Journal of Botany 50: 423–433. - Castellano M.A., Crabtree C.D., Mitchell D, Healy R.A. (2021)
Eight new Elaphomyces species (Elaphomycetaceae, Eurotiales,
Ascomycota) from eastern North America. Fungal Systematics and
Evolution 7: 113–131. - Castellano M.A., Dentinger B.T.M., Séné O., Elliott T.F., Truong C.,
Henkel T.W. (2016) New species of Elaphomyces (Elaphomycetaceae,
Eurotiales, Ascomycota) from tropical rainforests of Cameroon and
Guyana. IMA Fungus 7: 59–73. - Castellano M.A., Elliott T.F., Trappe J.M. (2018) Three new black
Elaphomyces species (Elaphomycetaceae, Eurotiales, Ascomycota)
from eastern North America with notes on selected European species.
Fungal Systematics and Evolution 1: 1–12. - Castellano M.A., Guerrero G.G., Jiménez J.G.,Trappe J.M. (2012b)
Elaphomyces appalachiensis and E. verruculosus sp. nov. (Ascomycota
Eurotiales, Elaphomycetaceae) from eastern North America. Revista
Mexicana de Micología 35: 17–22. - Castellano M.A., Henkel T.W., Miller S.L., Smith M.E., Aime M.C.
(2012c) New Elaphomyces species (Elaphomycetaceae, Eurotiales,
Ascomycota) from Guyana. Mycologia 104: 1244–1249. - Castellano M.A., Trappe J.M., Vernes K. (2011) Australian species of
Elaphomyces (Elaphomycetaceae, Eurotiales, Ascomycota). Australian
Systematic Botany 24: 32–57. - Castresana J. (2000) Selection of conserved blocks from multiple
alignments for their use in phylogenetic analysis. Molecular Biology
and Evolution 17: 540–552. - Darriba D., Taboada G.L., Doallo R., Posada D. (2012) jModelTest 2: more
models, new heuristics and parallel computing. Nature Methods 9: 772. - Gardes M., Bruns T.D. (1993) ITS primers with enhanced specificity for
basidiomycetes: application to the identification of mycorrhizae and
rusts. Molecular Ecology 2: 113–118. - Geyer C.J. (1991) Markov chain Monte Carlo maximum likelihood.
In: Keramidas E.M. (ed.) Computing science and statistics.
Proceedings of the 2third Symposium on the Interface. Interface
Foundation, Fairfax, VA, pp. 156–163. - Gouy M., Guindon S., Gascuel O. (2010) SeaView version 4: a
multiplatform graphical user interface for sequence alignment and
phylogenetic tree building. Molecular Biology and Evolution 27:
221–224. - Guindon S, Dufayard J.F., Lefort V., Anisimova M., Hordijk W., Gascuel O.
(2010) New algorithms and methods to estimate maximum-likelihood
phylogenies: assessing the performance of PhyML 3.0. Systematic
Biology 59: 307–321. - Guindon S., Gascuel O. (2003) A simple, fast, and accurate algorithm
to estimate large phylogenies by maximum likelihood. Systematic
Biology 52: 696–704. - Hatakeyama S, Orihara T. (2020) Elaphomyces asahimontanus
rediscovered from Japan 60 years after its original description [in
Japanese]. Truffology 3: 33–37 - Imai S. (1929) On the fungus-inhabiting Cordyceps and Elaphomyces in
Japan. Transactions of the Sapporo Natural History Society 11: 31–37. - Imai S. (1934) On a new species of Cordyceps parasitic on Elaphomyces
in Japan. Proceedings of the Imperial Academy (Japan) 10: 677–679. - Imai S. (1938) Third note on Elaphomyces and fungus-inhabiting
Cordyceps in Japan. Proceedings of the Imperial Academy (Japan) 14:
18–20. - Imai S. (1939) Fourth notes on Elaphomyces in Japan. Proceedings of the
Imperial Academy (Japan) 15: 146–147. - Izumitsu K., Hatoh K., Sumita T., Kitade Y., Morita A., Tanaka C., Gafur
A., Ohta A., Kawai M., Yamanaka T., Neda H., Ota Y. (2012) Rapid
and simple preparation of mushroom DNA directly from colonies and
fruiting bodies for PCR. Mycoscience 53: 396–401. - Japanese Society of Cordyceps Research (2014) An illustrated guide to
ecology of Japanese Cordyceps [in Japanese]. Seibundo-Shinkosha, Tokyo. - Katoh K., Standley D.M. (2013) MAFFT Multiple Sequence Alignment
Software Version 7: improvements in performance and usability.
Molecular Biology and Evolution 30: 772–780. - Kawamura S. (1914) Illustrations of Japanese fungi Vol. 4 [in Japanese]. The
Bureau of Forestry, the Ministry of Agriculture and Commerce, Tokyo. - Kobayasi Y. (1960) On the Elaphomyces found in Japan. Nagaoa 7:
35–50. - Lefort V., Longueville J.-E., Gascuel O. (2017) SMS: Smart Model
Selection in PhyML. Molecular Biology and Evolution 34: 2422–2424. - Lloyd C.G. (1916) Mycological notes 44. Index of the Mycological
Writings 5: 605–620. - Orihara T., Smith M.E., Shimomura N., Iwase K., Maekawa N. (2012)
Diversity and systematics of the sequestrate genus Octaviania in Japan:
two new subgenera and eleven new species. Persoonia 28: 85–112. - Paz A., Bellanger J., Lavoise C., Molia A., Ławrynowicz M., Larsson E.,
Ibarguren I.O., Jeppson M., Læssøe T., Sauvé M., Richard F., Moreau P.
(2017) The genus Elaphomyces (Ascomycota, Eurotiales): a ribosomal
DNA-based phylogeny and revised systematics of European ‘deer
truffles’. Persoonia 31: 197–239. - Ronquist F., Huelsenbeck J.P. (2003) MrBayes 3: Bayesian phylogenetic
inference under mixed models. Bioinformatics 19: 1572–1574. - Shimizu D. (1997) Illustrated vegetable wasps and plant worms in colour
[in Japanese]. Ie-no-hikari Association, Tokyo. - Stamatakis A. (2014) RAxML version 8: a tool for phylogenetic analysis
and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. - Umemura J. (1923) Nukata-gun Okazaki-mura no Yamato-tanpo-take to
Yamato-tsuchidango [in Japanese]. In: Aichi Prefecture (eds.): Aichi-ken Shiseki-meisho Ten-nen Kinen-butsu Chosa Hokoku No. 1, Aichi,
pp. 1–3. [ 梅村甚太郎 （1923） 額田郡岡崎村ノ大和たんぽたけト
大和つちだんご． 愛知県 編． 愛知県史蹟名勝天然紀念物調査
報告 第 1， pp. 1–3， 愛知県． ] - White T.J., Bruns T., Lee S., Taylor J. (1990) Amplification and direct
sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis
M.A., Gelfand D.H., Sninsky J.J., White T.J. (eds.), PCR Protocols: a
guide to methods and applications. Academic Press, USA, pp. 315–
322. - Yasuda A. (1919) Tochukaso-no-hanashi [in Japanese]. Rigakukai 16:
804–812. [ 安田篤 （1919） 冬蟲夏草の話． 理學界 16: 804–812.]