Balsamia oblonga (Helvellaceae), a new species from a subalpine forest in Japan

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Yamamoto K, Endo N, Ohmae M, Orihara T (2021) Truffology 4(1): 1–7
Submitted: 15 December 2020
Accepted: 1 March 2021
Published: 31 March 2021

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Abstract

Balsamia is a member of the family Helvellaceae, and all but one of the 25 species form subterranean truffle-like ascomata. This genus is broadly distributed across the Northern Hemisphere, i.e., Europe, North America, and Asia (China). However, this genus had not been found in Japan. In September 2013, an unidentified truffle-like fungus was collected in a subalpine forest in Gunma Prefecture, Japan. Morphological observations and molecular phylogenetic analyses revealed that this fungus belongs to the genus Balsamia. Here, we describe the new
species B. oblonga. This species is characterized by large, oblong ascospores (up to 33.5 μm) with a large length-to-width ratio (up to 3.3). Phylogenetic analyses resolved this species as a distinct species-level branch.

References

  1. Agerer R., Rambold G. (2004–2020) DEEMY – An information system
    for characterization and determination of ectomycorrhizae. Available at
    www.deemy.de (accessed on 8 Dec. 2020).

  2. Berkeley M.J. (1844) Notices of British fungi. Annals and Magazine of
    Natural History 13:340–360.

  3. Ceruti A., Bussetti L. (1962) Sulla simbiosi micorrizica tra tigli e Boletus
    subtomentosus, Russula grisea, Balsamia platyspora e Hysterangium
    clathroides. Allionia 8: 55–66.

  4. Crous P.W., Wingfield M.J., Schumacher R.K., Summerell B.A., Giraldo
    A., Gené J., Guarro J., Wanasinghe D.N., Hyde K.D., Camporesi E.,
    Gareth Jones E.B., Thambugala K.M., Malysheva E.F., Malysheva
    V.F., Acharya K., Álvarez J., Alvarado P., Assefa A., Barnes C.W.,
    Bartlett J.S., Blanchette R.A., Burgess T.I., Carlavilla J.R., Coetzee
    M.P., Damm U., Decock C.A., den Breeÿen A., de Vries B., Dutta
    A.K., Holdom D.G., Rooney-Latham S., Manjón J.L., Marincowitz
    S., Mirabolfathy M., Moreno G., Nakashima C., Papizadeh M.,
    Shahzadeh Fazeli S. A., Amoozegar M.A., Romberg M.K., Shivas R.G.,
    Stalpers J.A., Stielow B., Stukely M.J., Swart W.J., Tan Y.P., van der
    Bank M., Wood A.R., Zhang Y., Groenewald J.Z. (2014) Fungal Planet
    description sheets: 281–319. Persoonia 33: 212–289.

  5. Doğan H.H., Bozok F., Taşkın H. (2018) A new species of Barssia
    (Ascomycota, Helvellaceae) from Turkey. Turkish Journal of Botany
    42: 636–643.

  6. Gardes M., Bruns T.D. (1993) ITS primers with enhanced specificity for
    basidiomycetes: application to the identification of mycorrhizae and
    rusts. Molecular Ecology 2: 113–118.

  7. Gilkey H.M. (1939) Tuberales of North America. Oregon State
    Monographs: Studies in Botany 1: 1–63.

  8. Gilkey H.M. (1961) New species and revisions in the order Tuberales.
    Mycologia 53: 215–220.

  9. Hansen K., Schumacher T., Skrede I., Huhtinen S., Wang X.-H. (2019)
    Pindara revisited – evolution and generic limits in Helvellaceae.
    Persoonia 42: 186–204.

  10. Harkness H.W. (1899) Californian hypogæous fungi. Proceedings of the
    California Academy of Science, Third series, Botany 1: 241–286.

  11. Hawker L.E. (1954) British hypogeous fungi. Philosophical Transactions
    of the Royal Society B 237: 429–546.

  12. Hobbie E.A., Weber N.S., Trappe J.M. (2001) Mycorrhizal vs
    saprotrophic status of fungi: the isotopic evidence. New Phytologist
    150: 601–610.

  13. Izumitsu K., Hatoh K., Sumita T., Kitade Y., Morita A., Gafur A., Ohta
    A., Kawai M., Yamanaka T., Neda H., Ota Y., Tanaka C. (2012) Rapid
    and simple preparation of mushroom DNA directly from colonies and
    fruiting bodies for PCR. Mycoscience 53: 396–401.

  14. Kaounas V., Agnello C., Alvarado P., Slavova M. (2015) Barssia hellenica
    sp. nov. (Ascomycota, Pezizales), a new hypogeous species from Greece.
    Ascomycete.org 7: 213–219.

  15. Kobayasi Y. (1937) Phymatomyces, a new genus of the Tuberaceae.
    Journal of Japanese Botany 13: 912–914.

  16. Kumar S., Stecher G., Li M., Knyaz C., Tamura K. (2018) MEGA X:
    molecular evolutionary genetics analysis across computing platforms.
    Molecular Biology and Evolution 35: 1547–1549.

  17. Liu B., Tao K. (1990) New species and new records of hypogeous fungi
    from China III. Acta Mycologica Sinica 9: 25–30.

  18. Montecchi A., Sarasini M. (2000) Funghi ipogei d’Europa. Fondazione
    Centro Studi Micologici, Trento.

  19. Pegler D.N., Spooner B.M., Young T.W.K. (1993) British truffles: A
    revision of British hypogeous fungi. Royal Botanic Gardens, Kew.

  20. Petitberghien A. (1966) Note sur deux champignons hypogés. Bulletin
    Trimestriel de la Société Mycologique de France 82: 460–466.

  21. Schulzer S. (1870) Mykologische beobachtungen aus Nord-Ungarn im
    Herbste 1869. Verhandlungen der Zoologisch-Botanischen Gesellschaft
    in Österreich 20: 169–210.

  22. Silvestro D., Michalak I. (2012) raxmlGUI: a graphical front-end for
    RAxML Organisms, Diversity and Evolution 12: 335–337.

  23. Southworth D., Frank J.L., Castellano M.A., Smith M.E., Trappe J.M.
    (2018) Balsamia (sequestrate Helvellaceae, Ascomycota) in western
    North America. Fungal Systematics and Evolution 2: 11–36.

  24. Tedersoo L. Smith M.E. (2013) Lineages of ectomycorrhizal fungi
    revisited: Foraging strategies and novel lineages revealed by sequences
    from belowground. Fungal Biology Reviews 27: 83–99.

  25. Tulasne L.R., Tulasne C. (1843) Champignons hypogés de la famille des
    Lycoperdacées, observés dans les environs de Paris et les départements
    de la Vienne et d’lndre-et-Loire. Annales des Sciences Naturelles Série 2
    19: 373–381.

  26. Vilgalys R., Hester M. (1990) Rapid genetic identification and mapping
    of enzymatically amplified ribosomal DNA from several Cryptococcus
    species. Journal of Bacteriology 172: 4238–4246.

  27. Vittadini C. (1831) Monographia Tuberacearum. Mediolani.
  28. White T.J., Bruns T., Lee S., Taylor J. (1990) Amplification and direct
    sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis
    M.A., Gelfand D.H., Sninsky J.J., White T.J. (eds.) PCR protocols: a
    guide to methods and applications. Academic Press, USA, pp. 315–322.

  29. Xu Y.-Y., Guo L.-J., Li T., Fan L. (2018) Two new species of Barssia
    from China. Phytotaxa 374: 129–138.

  30. Xu Y.-Y., Yan X.-Y., Li T., Fan L. (2020) A taxonomic reassessment of
    the genus Balsamia from China. MycoKeys 67: 81–94.

  31. Yamamoto K., Ohmae M., Orihara T. (2019) First report of a hypogeous
    fungus, Pachyphlodes nemoralis (Pezizaceae) from subalpine forest in
    Japan. Truffology 2:1–5.

  32. Yamamoto K., Ohmae M., Orihara T. (2020) Metarhizium brachyspermum
    sp. nov. (Clavicipitaceae), a new species parasitic on Elateridae from
    Japan. Mycoscience 61: 37–42.